Interactions of GSK-3ß with mitochondrial permeability transition pore modulators during preconditioning: age-associated differences.
J Gerontol A Biol Sci Med Sci
; 68(4): 395-403, 2013 Apr.
Article
en En
| MEDLINE
| ID: mdl-23070879
ABSTRACT
Anesthetic preconditioning (APC) and ischemic preconditioning (IPC) are lost with normal aging. Here, we investigated age-related difference between phosphoglycogen synthase kinase-3beta (pGSK-3ß) and pGSK-3ß with modulators of mitochondrial permeability transition pore, including adenine nucleotide translocase (ANT), cyclophilin-D, or voltage-dependent anion channel. APC or IPC significantly increased pGSK-3ß in the young groups in both the cytosol and the mitochondria and also significantly increased pGSK-3ß in co-immunoprecipitates with ANT. Importantly, the level of cyclophilin-D in co-immunoprecipitates with ANT was significantly decreased in the young APC and IPC groups, but not in old rats. We also found that APC or IPC significantly prolonged mitochondrial permeability transition pore opening time in the young cardiomyocytes under oxidative stress, but not in the elderly. Attenuation of APC or IPC protection in the aging heart is associated with failure to reduce ANT-cyclophilin-D interactions and to decreased pGSK-3ß responsiveness of ANT, critical modulators of mitochondrial permeability transition pore.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Translocasas Mitocondriales de ADP y ATP
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Precondicionamiento Isquémico Miocárdico
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Ciclofilinas
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Glucógeno Sintasa Quinasa 3
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Proteínas de Transporte de Membrana Mitocondrial
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Canales Aniónicos Dependientes del Voltaje
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Mitocondrias Cardíacas
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
J Gerontol A Biol Sci Med Sci
Asunto de la revista:
GERIATRIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos