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In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay.
Boeckel, Daniel Gonçalves; Shinkai, Rosemary Sadami Arai; Grossi, Márcio Lima; Teixeira, Eduardo Rolim.
Afiliación
  • Boeckel DG; Private practice, Porto Alegre, Brazil. Electronic address: danielboeckel@yahoo.com.br.
  • Shinkai RS; Associate Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
  • Grossi ML; Associate Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
  • Teixeira ER; Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
Article en En | MEDLINE | ID: mdl-23146572
OBJECTIVE: To evaluate the cytotoxicity of hyaluronic acid (HA) on a tissue-engineered compound for bone grafting containing osteoblastic cells (OFCOL II), platelet-rich plasma (PRP) with or without thrombin (Thr), and hydroxyapatite (HP) by the MTT assay. STUDY DESIGN: Studied groups were formed as follows: (A) Cells + HA + PRP with Thr + hydroxyapatite (HP); (B) Cells + HA + PRP + HP; (C) Cells + HA + HP; (D) Cells + HP; (E) Cells + HA; (F) Cells + PRP with Thr; (G) Cells + PRP; and (H) Pure Dulbecco's modified Eagle's medium (DMEM) with 15% fetal bovine serum. A 2-way ANOVA and Tukey's test were applied for statistical analysis (P < .05). RESULTS: Results of cell viability for each group were as follows: A: 79%, B: 67%, C: 68%, D: 99%, E: 74%, G: 89%, F: 90%, and Group H: 100%. CONCLUSIONS: Results suggested a decrease in cell viability in the presence of HA.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Matriz Extracelular / Ácido Hialurónico Límite: Humans Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Matriz Extracelular / Ácido Hialurónico Límite: Humans Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos