Catecholamines can induce pulmonary remodeling in rats.
Cell Physiol Biochem
; 30(5): 1134-47, 2012.
Article
en En
| MEDLINE
| ID: mdl-23171784
ABSTRACT
BACKGROUND/AIMS:
Previously, we found that catecholamine (CA) infusion in rats induced pulmonary injury with edema and inflammation resembling acute lung injury in humans. Here, we examined effects of norepinephrine (NE) and of selective α- and ß-adrenergic agonists on the remodeling of pulmonary extracellular matrix.METHODS:
Eighty rats were infused over 8-72 h with NE, phenylephrine (PE), isoproterenol (ISO) or NaCl solution. We investigated mRNA expression of collagen, matrix metalloproteinase (MMP)-2, its tissue inhibitor (TIMP-2) and transforming growth factor (TGF)-ß isoforms in lung tissue. Additionally, lung histology, hemodynamic function and cardiac hypertrophy were evaluated.RESULTS:
After 72 h of infusion, lung histology showed beginning fibrosis and vascular hypertrophy. Collagen type I, MMP-2 and TIMP-2 mRNA expression were significantly elevated. All these effects were most pronounced with NE while PE and ISO induced weaker responses. TGF-ß mRNA expression was also elevated after 72 h, predominantly after PE infusion. Cardiac hypertrophy was most pronounced after ISO infusion.CONCLUSION:
CA infusion over 72 h may induce pulmonary remodeling. Mainly α-adrenergic but also ß-adrenergic mechanisms contribute to these processes. In contrast, cardiac hypertrophy is predominantly mediated by ß-adrenergic stimulation and hence, is considered to be a direct adrenergic effect rather than a consequence of pulmonary fibrosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenilefrina
/
Fibrosis Pulmonar
/
Norepinefrina
/
Matriz Extracelular
/
Isoproterenol
Límite:
Animals
Idioma:
En
Revista:
Cell Physiol Biochem
Asunto de la revista:
BIOQUIMICA
/
FARMACOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY