Frequent epigenetic silencing of PCDH10 by methylation in human colorectal cancer.
J Cancer Res Clin Oncol
; 139(3): 485-90, 2013 Mar.
Article
en En
| MEDLINE
| ID: mdl-23180019
ABSTRACT
PURPOSE:
Aberrant DNA methylation is common in cancer cells. Epigenetic alterations resulting in the loss of tumor suppression gene functions are frequently involved in tumor development and progression. Recently, methylation of PCDH10 was reported to be associated with multiple hematologic malignancies as well as some solid tumors. Whether the down-regulation of PCDH10 happens in CRC remains unknown.METHODS:
Methylation status of PCDH10 was evaluated by methylation-specific PCR analysis. The effects of PCDH10 re-expression were determined in growth, colony formation, cell cycle, and invasion assays.RESULTS:
In this study, we found that 100 % (8 of 8) of colorectal cancer cell lines were silenced for PCDH10, but not normal colorectal epithelial cells. Demethylation treatment confirmed that the reduced expression is associated closely with promoter methylation. Hyper-methylation of PCDH10 was also detected in 85 % of primary colorectal tumors, but not in adjacent normal colorectal tissues.CONCLUSIONS:
Our results suggest that PCDH10 is an important tumor suppression gene with key roles of suppressing cell proliferation, clonogenicity, and inhibiting cell invasion in the development of colorectal cancer. Thus, PCDH10 methylation may constitute a useful biomarker of colorectal cancer patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carcinoma
/
Neoplasias Colorrectales
/
Cadherinas
/
Metilación de ADN
/
Represión Epigenética
Límite:
Humans
Idioma:
En
Revista:
J Cancer Res Clin Oncol
Año:
2013
Tipo del documento:
Article
País de afiliación:
China