Successful establishment and evaluation of a new animal model for studying the hepatitis B virus YVDD mutant.
Arch Virol
; 158(4): 785-91, 2013 Apr.
Article
en En
| MEDLINE
| ID: mdl-23183771
ABSTRACT
The treatment of infection with lamivudine-resistant mutants of hepatitis B virus (HBV) with mutations in the YMDD motif has become a crucial issue in the clinic. In this work, the plasmids pcDNA3.1 (+)-HBV/C-YVDD and pcDNA3.1 (+)-HBV/C-YMDD were constructed and injected into BALB/c mice using a hydrodynamics-based procedure to investigate viral replication and expression of HBV lamivudine-resistant YVDD mutants in vivo. Compared with the YMDD group, HBsAg levels were higher in sera of mice in the YVDD group, but HBeAg levels were lower on day 1 after injection. Levels of HBcAg in hepatocytes were higher in the YVDD group on day 1, whereas the HBsAg levels were lower. The levels of HBV mRNA in the liver were higher in mice in the YVDD group on day 1 after injection. The results showed that injection with these plasmids resulted in efficient initiation of replication of HBV in mice and also suggested that the combined mutations in YVDD mutants could affect the replication process.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Hepatitis B
/
Modelos Animales de Enfermedad
/
Hepatitis B
/
Hepatitis Viral Animal
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
AT
/
AUSTRIA
/
ÁUSTRIA