Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy.
J Biophotonics
; 7(6): 392-400, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-23184354
ABSTRACT
Vibrational spectroscopy has long been used in bacterial identification with different levels of taxonomic discrimination but its true potential for intra-species differentiation remains poorly explored. Herein, both transmission Fourier-transform infrared (FTIR) and attenuated total reflectance (ATR)-FTIR spectroscopy are used to analyse E. coli strains that differ solely in their porin expression profile. In this previously unreported approach, the applicability of both FTIR-spectroscopy techniques is compared with the same collection of unique strains. ATR-FTIR spectroscopy proved to reliably distinguish between several E. coli porin mutants with an accuracy not replicated by FTIR in transmission mode (using previously optimized procedures). Further studies should allow the identification of the individual contribution of the single porin channel to the overall bacterial infrared spectrum and of molecular predictive patterns of porin alterations.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectroscopía Infrarroja por Transformada de Fourier
/
Porinas
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Mutación
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Biophotonics
Asunto de la revista:
BIOFISICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Portugal