Crystallization of domains involved in self-assembly of the S-layer protein SbsC.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 68(Pt 12): 1511-4, 2012 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-23192035
ABSTRACT
The Gram-positive bacterium Geobacillus stearothermophilus ATCC 12980 is completely covered with a two-dimensional crystalline monolayer composed of the S-layer protein SbsC. In order to complete the structure of the full-length protein, additional soluble constructs containing the crucial domains for self-assembly have been successfully cloned, expressed and purified. Crystals obtained from three different recombinant constructs yielded diffraction to 3.4, 2.8 and 1.5â
Å resolution. Native data have been collected.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Geobacillus stearothermophilus
/
Proteínas Bacterianas
/
Glicoproteínas de Membrana
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2012
Tipo del documento:
Article
País de afiliación:
Austria