Surgical technique for spinal cord delivery of therapies: demonstration of procedure in gottingen minipigs.
J Vis Exp
; (70): e4371, 2012 Dec 07.
Article
en En
| MEDLINE
| ID: mdl-23242422
ABSTRACT
This is a compact visual description of a combination of surgical technique and device for the delivery of (gene and cell) therapies into the spinal cord. While the technique is demonstrated in the animal, the procedure is FDA-approved and currently being used for stem cell transplantation into the spinal cords of patients with ALS. While the FDA has recognized proof-of-principle data on therapeutic efficacy in highly characterized rodent models, the use of large animals is considered critical for validating the combination of a surgical procedure, a device, and the safety of a final therapy for human use. The size, anatomy, and general vulnerability of the spine and spinal cord of the swine are recognized to better model the human. Moreover, the surgical process of exposing and manipulating the spinal cord as well as closing the wound in the pig is virtually indistinguishable from the human. We believe that the healthy pig model represents a critical first step in the study of procedural safety.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Espinal
/
Terapia Genética
/
Tratamiento Basado en Trasplante de Células y Tejidos
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2012
Tipo del documento:
Article