[Functional properties of the pOV13 plasmid as a vector for DNA cloning in a broad spectrum of gram negative bacteria]. / Funktsional'nye svoistva plazmidy pOV13 kak vektora dlia klonirovaniia DNK v shirokom kruge gramotritsatel'nykh bakterii.

ABSTRACT

The birepliconed plasmid pOV13 possesses all the properties of a vector for DNA cloning in a broad host range of bacterial cells. pOV13 is transfered by transformation and stably inherited by Escherichia coli, Brucella, Pseudomonas cells determining the resistance to streptomycin, tetrocycline and kanamycin in these bacteria. The plasmid pOV13 is a multicopy plasmid optimal in replication capacity (23kb). The plasmid carries single sites for some restriction endonucleases that are used for DNA cloning, including some restriction sites in antibiotic resistance genes. The examples of DNA cloning with the selection of recombinant clones by the insertional inactivation of kanamycin or tetracycline resistance and expression of the cloned DNAs are presented.