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Histological study of stem-like cells in human colon adenocarcinoma at different stages of the disease.
Florianova, L; Orain, M; Têtu, B; Doillon, C J.
Afiliación
  • Florianova L; Faculty of Medicine, Laval University, Quebec City, Canada.
Biotech Histochem ; 88(5): 222-34, 2013 Jul.
Article en En | MEDLINE | ID: mdl-23331188
ABSTRACT
The presence of stem-like cells in tumors reflects the invasive character of the disease; however, their identification is controversial. We investigated the distribution of CD133, CD44 and CD24 using histological sections and tissue microarrays (TMAs) of human colon adenocarcinoma obtained from patients with and without lymph node metastases and/or liver metastases. Immunohistochemical staining was combined with nuclear staining and evaluated quantitatively using image analysis software. Sections of normal colon mucosa, the primary tumor, lymph node, and liver also were analyzed qualitatively and compared to the quantitative method, which was more accurate. In most tissues, the expression of CD44 and CD24 was relatively low compared to CD133, with some variations. CD133 also was expressed in the normal colon mucosa and to a lesser degree in normal hepatic parenchyma. Liver metastases exhibited significantly greater CD133 staining compared to normal colon mucosa, primary tumor and lymph node metastases. Moreover, lymph node metastases obtained from patients with liver metastases expressed significantly greater CD133 staining than those obtained from patients without liver metastasis. Our data suggest that CD133 expression in lymph node metastases may be of value for prognosis of the disease.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Adenocarcinoma / Neoplasias del Colon Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biotech Histochem Asunto de la revista: HISTOCITOQUIMICA Año: 2013 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Adenocarcinoma / Neoplasias del Colon Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biotech Histochem Asunto de la revista: HISTOCITOQUIMICA Año: 2013 Tipo del documento: Article País de afiliación: Canadá