Algoriphagus shivajiensis sp. nov., isolated from Cochin back water, India.

ABSTRACT

Novel orange-pigmented, Gram-negative, rod-shaped, non-motile bacteria, designated strains NIO-S3(T) and NIO-S4, were isolated from a water sample collected from Cochin back waters, Thanneermukkom and Arookutty, Kerala, India. Both strains were positive for oxidase and catalase activities, and hydrolyzed gelatin and Tween 40. The predominant fatty acids were iso-C150, anteiso-C150, iso-C170 3OH, C161ω7c/C161ω6c (summed feature 3) and iso-C171ω9c/C160 10-methyl (summed feature 9), whereas MK-7 was the major respiratory quinone, and phosphatidylethanolamine, two unidentified phospholipids and one unidentified lipid were the only polar lipids. The DNA G+C content of the two strains was 43.7 and 43.6mol%, respectively. The 16S rRNA gene sequence analysis indicated that they were members of the genus Algoriphagus and closely related to Algoriphagus olei CC-Hsuan-617(T), Algoriphagus aquatilis A8-7(T), Algoriphagus aquaeductus LMG 24398(T) and Algoriphagus mannitolivorans DSM 15301(T), with pairwise sequence similarities of 96.8, 96.6, 96.2 and 96.2%, respectively. DNA-DNA hybridization between strains NIO-S3(T) and NIO-S4 showed a relatedness of 89%. Based on data from the current polyphasic study, the strains are proposed as a novel species of the genus Algoriphagus, for which the name Algoriphagus shivajiensis sp. nov. is proposed. The type strain of A. shivajiensis is NIO-S3(T) (=JCM 17885(T)=MTCC 11066(T)).