[Components of human complement system. Testing and isolation of C3 and C5]. / Komponenty sistemy komplementa cheloveka. Testirovanie i vydelenie C3 i C5.
Biokhimiia
; 55(2): 276-84, 1990 Feb.
Article
en Ru
| MEDLINE
| ID: mdl-2340315
ABSTRACT
Modified reagents for testing the hemolytic activity of human complement components, C3 and C5, have been obtained. These reagents were obtained by treatment of human blood serum pools with a saturated solution of KBr (reagent R3) or 2 M KSCN and denaturated yeasts (reagent R5). These reagents were found to be rich in the serum factor obtained through the use of DEAE-cellulose DE-52 and containing the active component of the complement (C4). To test the sensitivity and specificity of the above reagents, components C3 and C5 were purified. After this procedure these components emerged as hemolytically active, electrophoretically and immunophoretically homogeneous components, C3 and C5. DEAE-cellulose DE-52, DEAE-Sephacel, Hydroxylapatite and Ultra-gel AcA-34 were used consecutively as purification agents. The activity yields of components C3 and C5 with regard to the initial serum levels were 31% and 18%, respectively.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Complemento C3
/
Complemento C5
Límite:
Humans
Idioma:
Ru
Revista:
Biokhimiia
Año:
1990
Tipo del documento:
Article