[Identification and characterization of microRNA involving in malignant pleural mesothelioma]. / Identificazione e caratterizzazione dei microRNA coinvolti nel mesotelioma pleurico maligno.
G Ital Med Lav Ergon
; 34(3 Suppl): 552-4, 2012.
Article
en It
| MEDLINE
| ID: mdl-23405713
ABSTRACT
One of the research areas of modern medicine is to work on the identification of biological markers, such as biomolecular ones, for neoplastic diseases from occupational origin. MiRNA, short RNA no-codifing sequences, are recently identified such as diagnostic markers in several type of cancer. For this reason, the aim of our study is to analyze the possible role of miRNA in malignant pleural mesothelioma, a rare and aggressive tumor with a strong resistance to conventional therapies and poor prognosis. Total RNA, containing also miRNA, was extracted, and RNA was retro-transcripted with specific primers. Then, miRNA expression was tested using real-time PCR method and particular probes for each miRNA. The RNU6B was used such as housekeeping gene, for data normalization. This work represents the first step for the identification of a specific miRNA pattern for MPM, which will be useful in the diagnosis of MPM and for a personalized therapeutic treatment.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Pleurales
/
MicroARNs
/
Mesotelioma
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
It
Revista:
G Ital Med Lav Ergon
Asunto de la revista:
MEDICINA OCUPACIONAL
Año:
2012
Tipo del documento:
Article