Oxidative decarboxylation of mandelic acid derivative by recombinant Escherichia coli: a novel method of ethyl vanillin synthesis.
Biotechnol Lett
; 35(6): 921-7, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23430129
ABSTRACT
The benzoylformate decarboxylase gene (mdlC) from Pseudomonas putida was expressed in Escherichia coli BL21(DE3). The recombinant strain together with E. coli/pET30a-mdlB converted (S)-3-ethoxy-4-hydroxymandelic acid (S-EMA) into ethyl vanillin without ethyl vanillin degradation. 4 g ethyl vanillin/l was obtained from 10 g EMA/l within 12 h at 30 °C. This is the first report on the biotransformation of (S)-EMA to ethyl vanillin.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Benzaldehídos
/
Carboxiliasas
/
Pseudomonas putida
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Escherichia coli
/
Ingeniería Metabólica
/
Ácidos Mandélicos
Idioma:
En
Revista:
Biotechnol Lett
Año:
2013
Tipo del documento:
Article
País de afiliación:
China