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TRACER: a resource to study the regulatory architecture of the mouse genome.
Chen, Chao-Kung; Symmons, Orsolya; Uslu, Veli Vural; Tsujimura, Taro; Ruf, Sandra; Smedley, Damian; Spitz, François.
Afiliación
  • Chen CK; European Bioinformatics Institute - European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
BMC Genomics ; 14: 215, 2013 Apr 02.
Article en En | MEDLINE | ID: mdl-23547943
BACKGROUND: Mammalian genes are regulated through the action of multiple regulatory elements, often distributed across large regions. The mechanisms that control the integration of these diverse inputs into specific gene expression patterns are still poorly understood. New approaches enabling the dissection of these mechanisms in vivo are needed. RESULTS: Here, we describe TRACER (http://tracerdatabase.embl.de), a resource that centralizes information from a large on-going functional exploration of the mouse genome with different transposon-associated regulatory sensors. Hundreds of insertions have been mapped to specific genomic positions, and their corresponding regulatory potential has been documented by analysis of the expression of the reporter sensor gene in mouse embryos. The data can be easily accessed and provides information on the regulatory activities present in a large number of genomic regions, notably in gene-poor intervals that have been associated with human diseases. CONCLUSIONS: TRACER data enables comparisons with the expression pattern of neighbouring genes, activity of surrounding regulatory elements or with other genomic features, revealing the underlying regulatory architecture of these loci. TRACER mouse lines can also be requested for in vivo transposition and chromosomal engineering, to analyse further regions of interest.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma / Bases de Datos Genéticas Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2013 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma / Bases de Datos Genéticas Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2013 Tipo del documento: Article Pais de publicación: Reino Unido