Neopterin negatively regulates expression of ABCA1 and ABCG1 by the LXRα signaling pathway in THP-1 macrophage-derived foam cells.
Mol Cell Biochem
; 379(1-2): 123-31, 2013 Jul.
Article
en En
| MEDLINE
| ID: mdl-23564066
ABSTRACT
To investigate the effects of neopterin on ABCA1 expression and cholesterol efflux in human THP-1 macrophage-derived foam cells, and to explore the role of the liver X receptor alpha (LXRα) involved. In the present study, THP-1 cells were pre-incubated with ox-LDL to become foam cells. The protein and mRNA expression were examined by Western blot assays and real-time quantitative PCR, respectively. Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content. Neopterin decreased ABCA1 expression and cholesterol efflux in a time- and concentration-dependent manner in THP-1 macrophage-derived foam cells, and the LXRα siRNA can reverse the inhibitory effects induced by neopterin. Neoterin has a negative regulation on ABCA1 expression via the LXRα signaling pathway, which suggests the aggravated effects of neopterin on atherosclerosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transportadoras de Casetes de Unión a ATP
/
Neopterin
/
Receptores Nucleares Huérfanos
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Transportador 1 de Casete de Unión a ATP
/
Células Espumosas
Límite:
Humans
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2013
Tipo del documento:
Article
País de afiliación:
China