Characterization of TGFß signaling during tail regeneration in the leopard Gecko (Eublepharis macularius).
Dev Dyn
; 242(7): 886-96, 2013 Jul.
Article
en En
| MEDLINE
| ID: mdl-23592270
ABSTRACT
INTRODUCTION:
The transforming growth factor beta (TGFß)/activin signaling pathway has a number of documented roles during wound healing and is increasingly appreciated as an essential component of multi-tissue regeneration that occurs in amphibians and fish. Among amniotes (reptiles and mammals), less is known due in part to the lack of an appropriate model organism capable of multi-tissue regeneration. The leopard gecko Eublepharis macularius is able to spontaneously, and repeatedly, regenerate its tail following tail loss. We examined the expression and localization of several key components of the TGFß/activin signaling pathway during tail regeneration of the leopard gecko.RESULTS:
We observed a marked increase in phosphorylated Smad2 expression within the regenerate blastema indicating active TGFß/activin signaling. Interestingly, during early regeneration, TGFß1 expression is limited whereas activin-ßA is strongly upregulated. We also observe the expression of EMT transcription factors Snail1 and Snail2 in the blastema.CONCLUSIONS:
Combined, these observations provide strong support for the importance of different TGFß ligands during multi-tissue regeneration and the potential role of TGFß/activin-induced EMT programs during this process.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cola (estructura animal)
/
Factor de Crecimiento Transformador beta
/
Lagartos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Dev Dyn
Asunto de la revista:
ANATOMIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Canadá