Detection and quantification of Leishmania braziliensis in ectoparasites from dogs.
Vet Parasitol
; 196(3-4): 506-8, 2013 Sep 23.
Article
en En
| MEDLINE
| ID: mdl-23601843
ABSTRACT
American cutaneous leishmaniasis (ACL) is a disease caused by different species of Leishmania protozoa, Leishmania braziliensis being the main species found in Brazil. In this study, two rural areas in Pernambuco, northeastern Brazil, where ACL is endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) and tested using a conventional PCR and a quantitative real time PCR. A total of 117 ectoparasites were collected, being 50 (42.74%) of them positive for L. braziliensis (in at least one PCR protocol), with a mean parasite load of 14.14 fg/µL. Furthermore, 46 (92.00%) positive ectoparasites were collected from positive dogs and 4 (8.00%) from negative ones. This study reports the detection of L. braziliensis DNA in ectoparasites, but does not prove their vector competence. Certainly, experimental transmission studies are necessary to assess their role, if any, in the transmission of Leishmania parasites to dogs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Garrapatas
/
Leishmania braziliensis
/
Enfermedades de los Perros
/
Siphonaptera
/
Phthiraptera
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Vet Parasitol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil