Enzymatic product-mediated stabilization of CdS quantum dots produced in situ: application for detection of reduced glutathione, NADPH, and glutathione reductase activity.
Anal Chem
; 85(11): 5542-6, 2013 Jun 04.
Article
en En
| MEDLINE
| ID: mdl-23656502
ABSTRACT
Glutathione is the most abundant nonprotein molecule in the cell and plays an important role in many biological processes, including the maintenance of intracellular redox states, detoxification, and metabolism. Furthermore, glutathione levels have been linked to several human diseases, such as AIDS, Alzheimer disease, alcoholic liver disease, cardiovascular disease, diabetes mellitus, and cancer. A novel concept in bioanalysis is introduced and applied to the highly sensitive and inexpensive detection of reduced glutathione (GSH), over its oxidized form (GSSG), and glutathione reductase (GR) in human serum. This new fluorogenic bioanalytical system is based on the GSH-mediated stabilization of growing CdS nanoparticles. The sensitivity of this new assay is 5 pM of GR, which is 3 orders of magnitude better than other fluorogenic methods previously reported.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sulfuros
/
Compuestos de Cadmio
/
Disulfuro de Glutatión
/
Puntos Cuánticos
/
Glutatión
/
Glutatión Reductasa
/
NADP
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Chem
Año:
2013
Tipo del documento:
Article
País de afiliación:
España