Saliva improves Streptococcus mitis protective effect on human gingival fibroblasts in presence of 2-hydroxyethyl-methacrylate.
J Mater Sci Mater Med
; 24(8): 1977-83, 2013 Aug.
Article
en En
| MEDLINE
| ID: mdl-23670604
ABSTRACT
This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saliva
/
Streptococcus mitis
/
Fibroblastos
/
Encía
/
Metacrilatos
Límite:
Humans
Idioma:
En
Revista:
J Mater Sci Mater Med
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Italia