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Applicability of DiversiLab repetitive sequence-based PCR method in epidemiological typing of enterohemorrhagic Escherichia coli (EHEC).
Lukinmaa-Åberg, Susanna; Horsma, Jenni; Pasanen, Tanja; Mero, Sointu; Aulu, Laura; Vaara, Martti; Siitonen, Anja; Antikainen, Jenni.
Afiliación
  • Lukinmaa-Åberg S; Bacteriology Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare THL, Helsinki, Finland.
Foodborne Pathog Dis ; 10(7): 632-8, 2013 Jul.
Article en En | MEDLINE | ID: mdl-23692078
Enterohemorrhagic Escherichia coli (EHEC) causes diarrhea, often with severe complications. Rapid and discriminatory typing of EHEC using advanced molecular methods is needed for determination of the genetic relatedness of clones responsible for foodborne outbreaks and for finding out the transmission sources of the outbreaks. This study evaluated the potential of DiversiLab, a semiautomated repetitive sequence-based polymerase chain reaction method for the genotyping of EHEC strains. A set of 52 EHEC strains belonging to 15 O:H serotypes was clustered into 10 DiversiLab groups. All of the O157 strains and one O55 strain were classified into the same group based on a 90% similarity threshold. The other serotypes were classified to their own DiversiLab group, with the exception of one R:H(-) strain that was grouped with O5:H(-) strains. In addition, O26 and O111 strains were grouped together but ultimately subdivided according to their O-serotypes based on a 95% similarity threshold. The O104 strain, which was associated with a major outbreak of hemolytic uremic syndrome in Germany in May 2011, was also classified independently. The DiversiLab performed well in identifying isolates, but the discriminatory power of the repetitive sequence-based polymerase chain reaction method was lower than that of pulsed-field gel electrophoresis. Analysis of 15 enteropathogenic E. coli (EPEC) strains revealed that some EPEC strains clustered together with EHEC strains. Therefore, the DiversiLab system cannot be used to discriminate between these pathogroups. In conclusion, DiversiLab is a rapid and easy system for the primary exclusion of unrelated EHEC strains based on their serotypes, but more discriminatory methods, such as pulsed-field gel electrophoresis, are needed for accurate typing of the EHEC strains.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secuencias Repetitivas de Ácidos Nucleicos / Técnicas de Tipificación Bacteriana / Infecciones por Escherichia coli / Escherichia coli Enterohemorrágica / Síndrome Hemolítico-Urémico Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secuencias Repetitivas de Ácidos Nucleicos / Técnicas de Tipificación Bacteriana / Infecciones por Escherichia coli / Escherichia coli Enterohemorrágica / Síndrome Hemolítico-Urémico Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Estados Unidos