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Development of micellar reactive oxygen species assay for photosafety evaluation of poorly water-soluble chemicals.
Seto, Yoshiki; Kato, Masashi; Yamada, Shizuo; Onoue, Satomi.
Afiliación
  • Seto Y; Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
Toxicol In Vitro ; 27(6): 1838-46, 2013 Sep.
Article en En | MEDLINE | ID: mdl-23727251
A reactive oxygen species (ROS) assay was previously developed for photosafety assessment; however, the phototoxic potential of some chemicals cannot be evaluated because of their limited aqueous solubility. The present study was undertaken to develop a new micellar ROS (mROS) assay system for poorly water-soluble chemicals using a micellar solution of 0.5% (v/v) Tween 20 for solubility enhancement. In repeated mROS assay, intra- and inter-day precisions (coefficient of variation) were found to be below 11%, and the Z'-factors for singlet oxygen and superoxide suggested a large separation band between positive and negative standards. The ROS and mROS assays were applied to 65 phototoxins and 18 non-phototoxic compounds for comparative purposes. Of all 83 chemicals, 25 were unevaluable in the ROS assay due to poor solubility, but only 2 were in the mROS assay. Upon mROS assay on these model chemicals, the individual specificity was 76.5%, and the positive and negative predictivities were found to be 93.9% and 86.7%, respectively. The mROS assay provided 2 false negative predictions, although negative predictivity for the ROS assay was found to be 100%. Considering the pros and cons of these assays, strategic combined use of the ROS and mROS assays might be efficacious for reliable photosafety assessment with high applicability and predictivity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Fármacos Fotosensibilizantes / Superóxidos / Oxígeno Singlete Tipo de estudio: Prognostic_studies Idioma: En Revista: Toxicol In Vitro Asunto de la revista: TOXICOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Fármacos Fotosensibilizantes / Superóxidos / Oxígeno Singlete Tipo de estudio: Prognostic_studies Idioma: En Revista: Toxicol In Vitro Asunto de la revista: TOXICOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido