Chronic Mycoplasma conjunctivitis in house finches: host antibody response and M. gallisepticum VlhA expression.
Vet Immunol Immunopathol
; 154(3-4): 129-37, 2013 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-23764469
ABSTRACT
Previous studies have shown that house finch field isolates of Mycoplasma gallisepticum (MG) vary in virulence and ability to induce an antibody response. After experimental inoculation, MG causes persistent, severe disease in a subset of individuals. In this study, we further characterized MG infection using five field isolates, with an emphasis on chronically diseased birds. After experimental inoculation of house finches, MG load was measured by quantitative PCR and anti-MG antibody responses were measured by ELISAs. Birds with chronic disease had significantly higher pathogen loads and antibody responses than did birds without chronic disease. Using a monoclonal antibody (MAb86) specific for a variant of the MG VlhA adhesin and immunodominant surface protein, we show that VlhA expression differs among MG isolates in this study, and that in vivo VlhA changes occur in house finches infected with MG. Overall, our results suggest that chronic MG disease has a strong pathogen-mediated component.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Enfermedades de las Aves
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Mycoplasma gallisepticum
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Pinzones
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Anticuerpos Antibacterianos
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Infecciones por Mycoplasma
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos