8-hydroxyguanine in urine and serum as an oxidative stress marker: effects of diabetes and aging.
J UOEH
; 35(2): 119-27, 2013 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-23774655
ABSTRACT
8-Hydroxydeoxyguanosine (8-OH-dG) is the most extensively analyzed oxidative stress marker. Recently, 8-hydroxyguanine (free base 8-OH-Gua) has been recognized as an oxidative stress marker. To verify the usefulness of 8-OH-Gua, the 8-OH-dG and 8-OH-Gua levels in the urine and the 8-OH-Gua levels in the serum of type 2 diabetic model animals, db/db mice, were measured as oxidative stress markers by a column switching HPLC-system coupled to an electrochemical detector. The urinary 8-OH-Gua and 8-OH-dG levels in db/db mice (7-26 weeks old) were significantly higher than those in control (db/m+) mice. The 8-OH-Gua levels in the serum of the db/db mice were also about 2-fold higher than those in the control mice at 26 weeks of age. In addition, the urinary levels of 8-OH-dG and 8-OH-Gua increased with age (9-26 weeks). A significant positive correlation was obtained between the 8-OH-dG and 8-OH-Gua levels in urine. Although no difference was observed in the 8-OH-dG levels in the liver and kidney DNA between the diabetic and control mice, these results suggested that urinary 8-OH-dG and free base 8-OH-Gua in urine or serum may be good biomarkers of oxidative stress.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Envejecimiento
/
Biomarcadores
/
Estrés Oxidativo
/
Diabetes Mellitus Experimental
/
Guanina
Límite:
Animals
Idioma:
En
Revista:
J UOEH
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón