Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran.
Opt Express
; 21(12): 14662-73, 2013 Jun 17.
Article
en En
| MEDLINE
| ID: mdl-23787655
ABSTRACT
A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dextranos
/
Arabidopsis
/
Electroporación
/
Epidermis de la Planta
/
Colorantes Fluorescentes
/
Rayos Láser
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos