Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7.
Biosens Bioelectron
; 49: 492-8, 2013 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-23811484
ABSTRACT
In this study, a low-cost and robust impedimetric immunosensor based on gold nanoparticles modified free-standing graphene paper electrode for rapid and sensitive detection of Escherichia coli O157H7 (E. coli O157H7) was developed. Graphene paper was prepared by chemical reduction of graphene oxide paper obtained from vacuum filtration method. Scanning electron microscope, Raman spectroscopy and X-ray diffraction techniques were employed to investigate the surface morphology and crystal structure of the prepared graphene paper. The gold nanoparticles were grown on the surface of graphene paper electrode by one-step electrodeposition technique. The immobilization of anti-E. coli O157H7 antibodies on paper electrode were performed via biotin-streptavidin system. Electrochemical impedance spectroscopy was used to detect E. coli O157H7 captured on the paper electrode. Results show that the developed paper immunosensor possesses greatly enhanced sensing performance, such as wide linear range (1.5 × 10(2)-1.5 × 10(7) cfu mL(-1)), low detection limit (1.5 × 10(2) cfu mL(-1)), and excellent specificity. Furthermore, flexible test demonstrate the graphene paper based sensing device has high tolerability to mechanical stress. The strategy of structurally integrating metal nanomaterials, graphene paper, and biorecognition molecules would provide new insight into design of flexible immunosensors for routine sensing applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Escherichia coli O157
/
Nanopartículas
/
Anticuerpos Inmovilizados
/
Oro
/
Grafito
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2013
Tipo del documento:
Article