[Clustering analysis of Mycobacterium tuberculosis using the JATA(12)-VNTR system for molecular epidemiological surveillance in broad areas of Japan].
Kekkaku
; 88(4): 393-8, 2013 Apr.
Article
en Ja
| MEDLINE
| ID: mdl-23819314
ABSTRACT
OBJECTIVES:
Japan Anti-Tuberculosis Association (JATA) (12)-variable numbers of tandem repeats (VNTR) is a standard method for genotyping of clinical isolates of Mycobacterium tuberculosis in Japan. As a model study for nationwide surveillance, this study aimed to describe the tendency and frequency of genotypes of M. tuberculosis in a large number of clinical samples.METHODS:
Clinical isolates of M. tuberculosis (n = 1,778) were obtained from patients with tuberculosis in 3 areas, i.e., Osaka City, Osaka Prefecture, and Kobe City, during 2007 and 2008. The samples were analyzed using JATA (12)-VNTR. All genotypes were subjected to clustering analysis. RESULTS ANDDISCUSSION:
In total, 1,086 (61.1%) isolates showed clustering. The most common clusters were composed of 3 members. Such clusters were considered to reflect either actual transmission or low discriminatory power of JATA (12)-VNTR. Several prevalent JATA(12)-VNTR genotypes formed large clusters and were discussed in relation with epidemiological findings of other studies. The findings of this study will aid in the construction of an effective genotyping-based surveillance system of M. tuberculosis, through improvement of interpretation of VNTR types, observation of certain particular strains in an area, and efficient detection of unidentified outbreaks.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tuberculosis
/
Análisis por Conglomerados
/
Epidemiología Molecular
/
Repeticiones de Minisatélite
/
Técnicas de Genotipaje
/
Mycobacterium tuberculosis
Tipo de estudio:
Prognostic_studies
/
Screening_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Asia
Idioma:
Ja
Revista:
Kekkaku
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón