Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
J Vet Sci
; 14(4): 491-4, 2013.
Article
en En
| MEDLINE
| ID: mdl-23820221
ABSTRACT
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Ovejas
/
Actinomycetales
/
Infecciones por Actinomycetales
/
Enfermedades de los Bovinos
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Colorantes Fluorescentes
/
Enfermedades de los Caballos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Vet Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2013
Tipo del documento:
Article