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[DNA extraction from bones and teeth using AutoMate Express forensic DNA extraction system].
Gao, Lin-Lin; Xu, Nian-Lai; Xie, Wei; Ding, Shao-Cheng; Wang, Dong-Jing; Ma, Li-Qin; Li, You-Ying.
Afiliación
  • Gao LL; Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310004, China. myhappyfamily_gao@163.com
Fa Yi Xue Za Zhi ; 29(2): 127-9, 2013 Apr.
Article en Zh | MEDLINE | ID: mdl-23930509
OBJECTIVE: To explore a new method in order to extract DNA from bones and teeth automatically. METHODS: Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system. RESULTS: DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method. CONCLUSION: AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diente / Huesos / ADN / Dermatoglifia del ADN / Medicina Legal Tipo de estudio: Guideline Límite: Humans Idioma: Zh Revista: Fa Yi Xue Za Zhi Asunto de la revista: JURISPRUDENCIA Año: 2013 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diente / Huesos / ADN / Dermatoglifia del ADN / Medicina Legal Tipo de estudio: Guideline Límite: Humans Idioma: Zh Revista: Fa Yi Xue Za Zhi Asunto de la revista: JURISPRUDENCIA Año: 2013 Tipo del documento: Article País de afiliación: China Pais de publicación: China