MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis.
J Neurooncol
; 115(2): 179-88, 2013 Nov.
Article
en En
| MEDLINE
| ID: mdl-23943502
ABSTRACT
Previous studies reported that miR-29c is significantly downregulated in several tumors. However, little is known about the effect and molecular mechanisms of action of miR-29c in human glioma. Using quantitative RT-PCR, we demonstrated that miR-29c was significantly downregulated in glioma cell lines and human primary glioma tissues, compared to normal human astrocytes and matched non-tumor associated tissues (P < 0.05, χ(2) test). Overexpression of miR-29c dramatically reduced the proliferation and caused cessation of cell cycle. The reduced cell proliferation is due to G1 phase arrest as cyclin D1 and cyclin E are diminished whereas p27 and p21 are upregulated. We further demonstrated that miR-29c overexpression suppressed the glioma cell migration and invasion abilities by targeting MMP-2. In addition, we also found that overexpression of miR-29c sharply inhibited angiogenesis, which correlated with down-regulation of VEGF. The data indicate that miR-29c may be a tumor suppressor involved in the progression of glioma.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
Movimiento Celular
/
MicroARNs
/
Proliferación Celular
/
Glioma
/
Neovascularización Patológica
Límite:
Humans
Idioma:
En
Revista:
J Neurooncol
Año:
2013
Tipo del documento:
Article