Biphasic myosin II light chain activation during clot retraction.
Thromb Haemost
; 110(6): 1215-22, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-23965920
ABSTRACT
Clot retraction is an essential step during primary haemostasis, thereby promoting thrombus stability and wound healing. Integrin αIIbß3 plays a critical role in clot retraction, by inducing acto-myosin interactions that allow platelet cytoskeleton reorganisation. However, the signalling pathways that lead to clot retraction are still misunderstood. In this study, we report the first data on the kinetics of myosin II light chain (MLC) phosphorylation during clot retraction. We found an early phosphorylation peak followed by a second peak. By using specific inhibitors of kinases and small G proteins, we showed that MLC kinase (MLCK), RhoA/ROCK, and Rac-1 were involved in clot retraction and in the early MLC phosphorylation peak. Only Rac-1 and actin polymerisation, controlled by outside-in signalling, were crucial to the second MLC phosphorylation peak.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Citoesqueleto de Actina
/
Complejo GPIIb-IIIa de Glicoproteína Plaquetaria
/
Cadenas Ligeras de Miosina
/
Miosina Tipo II
Límite:
Humans
Idioma:
En
Revista:
Thromb Haemost
Año:
2013
Tipo del documento:
Article