Prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes among blood and urinary Escherichia coli isolates.
J Med Microbiol
; 62(Pt 11): 1707-1713, 2013 Nov.
Article
en En
| MEDLINE
| ID: mdl-23988630
ABSTRACT
A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistance genes. Eighteen of the 1878 clinical E. coli isolates were fosfomycin resistant, of which six were fosA3 positive and two were positive for another fosA variant (designated fosKP96). No isolates had the fosC2 gene. The clones of the eight isolates were diverse sequence type (ST) 95 (nâ=â2), ST118 (nâ=â1), ST131 (nâ=â1), ST617 (nâ=â1), ST648 (nâ=â1), ST1488 (nâ=â1) and ST2847 (nâ=â1). In the isolates, fosA3 and blaCTX-M genes were co-harboured on conjugative plasmids with F2A-B- (nâ=â2), N (nâ=â1), F-A-B1 and N (nâ=â1) and untypable (nâ=â2) replicons. Both fosKP96-carrying plasmids belonged to replicon N. RFLP analysis showed that the two F2A-B- plasmids carrying fosA3 and blaCTX-M-3 genes shared the same pattern. Complete sequencing of one of the two F2A-B- plasmids, pFOS-HK151325 (69â768 bp) demonstrated it to be >99â% identical to the previously sequenced plasmid pHK23a originating from a pig E. coli isolate in the same region. This study demonstrated the dissemination of fosA3 genes in diverse E. coli clones on multiple blaCTX-M-carrying plasmid types, of which F2A-B- plasmids closely related to pHK23a were shared by isolates from human and animal sources.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones Urinarias
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Bacteriemia
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Farmacorresistencia Bacteriana
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Escherichia coli
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Infecciones por Escherichia coli
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Fosfomicina
/
Antibacterianos
Tipo de estudio:
Prevalence_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Med Microbiol
Año:
2013
Tipo del documento:
Article