Novel agents for enzymatic and fungal hydrolysis of stevioside.
Braz J Microbiol
; 40(2): 367-72, 2009 Apr.
Article
en En
| MEDLINE
| ID: mdl-24031374
ABSTRACT
A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae) and enzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield).
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Braz J Microbiol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
BR
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BRASIL
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BRASILE
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BRAZIL
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BRESIL