Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells.
FEBS Lett
; 260(1): 127-30, 1990 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-2404793
ABSTRACT
We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata. The sequence covers the whole transcribed region as well as the 5'- and 3'-untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G-50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plantas Medicinales
/
Genes de Plantas
/
Concanavalina A
/
Escherichia coli
/
Vectores Genéticos
/
Fabaceae
Idioma:
En
Revista:
FEBS Lett
Año:
1990
Tipo del documento:
Article
País de afiliación:
Japón