Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic ß-cells.
Sci Rep
; 3: 2863, 2013 Oct 04.
Article
en En
| MEDLINE
| ID: mdl-24091640
ABSTRACT
Diabetes is caused by the loss or dysfunction of insulin-secreting ß-cells in the pancreas. ß-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as ß-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary ß-cells in direct contact with 'synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of ß-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced ß-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Materiales Biomiméticos
/
Células Secretoras de Insulina
/
Microesferas
Límite:
Animals
Idioma:
En
Revista:
Sci Rep
Año:
2013
Tipo del documento:
Article