Molecular epidemiology of Clostridium difficile at a medical center in Taiwan: persistence of genetically clustering of Aâ»B⺠isolates and increase of AâºB⺠isolates.
PLoS One
; 8(10): e75471, 2013.
Article
en En
| MEDLINE
| ID: mdl-24116048
ABSTRACT
INTRODUCTION:
We investigated the changing trend of various toxigenic Clostridium difficile isolates at a 3 500-bed hospital in Taiwan. Genetic relatedness and antimicrobial susceptibility of toxigenic C. difficile isolates were also examined.METHODS:
A total of 110 non-repeat toxigenic C. difficile isolates from different patients were collected between 2002 and 2007. Characterization of the 110 toxigenic isolates was performed using agar dilution method, multilocus variable-number tandem-repeat analysis (MLVA) genotyping, tcdC genotyping, and toxinotyping.RESULTS:
Among the 110 toxigenic isolates studied, 70 isolates harbored tcdA and tcdB (AâºBâº) and 40 isolates harbored tcdB only (Aâ»Bâº). The annual number of AâºB⺠isolates considerably increased over the 6-year study (Pâ=â0.055). A total of 109 different MLVA genotypes were identified, in which AâºB⺠isolates and Aâ»B⺠isolates were differentiated into two genetic clusters with similarity of 17.6%. Twenty-four (60%) of the 40 Aâ»B⺠isolates formed a major cluster, MLVA-group 1, with a similarity of 85%. Seven (6.4%) resistant isolates were identified, including two metronidazole-resistant and five vancomycin-resistant isolates.CONCLUSIONS:
This study indicated a persistence of a MLVA group 1 Aâ»B⺠isolates and an increase of AâºB⺠isolates with diverse MLVA types. Moreover, C. difficile isolates with antimicrobial resistance to metronidazole or vancomycin were found to have emerged. Continuous surveillance is warranted to understand the recent situation and control the further spread of the toxigenic C. difficile isolates, especially among hospitalized patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clostridioides difficile
/
Farmacorresistencia Bacteriana
Tipo de estudio:
Prognostic_studies
/
Screening_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Taiwán