Induction of a chloracne phenotype in an epidermal equivalent model by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on aryl hydrocarbon receptor activation and is not reproduced by aryl hydrocarbon receptor knock down.
J Dermatol Sci
; 73(1): 10-22, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24161567
ABSTRACT
BACKGROUND:
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent activator of the aryl hydrocarbon receptor (AhR) and causes chloracne in humans. The pathogenesis and role of AhR in chloracne remains incompletely understood.OBJECTIVE:
To elucidate the mechanisms contributing to the development of the chloracne-like phenotype in a human epidermal equivalent model and identify potential biomarkers.METHODS:
Using primary normal human epidermal keratinocytes (NHEK), we studied AhR activation by XRE-luciferase, AhR degradation and CYP1A1 induction. We treated epidermal equivalents with high affinity TCDD or two non-chloracnegens ß-naphthoflavone (ß-NF) and 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE). Using Western blotting and immunochemistry for filaggrin (FLG), involucrin (INV) and transglutaminase-1 (TGM-1), we compared the effects of the ligands on keratinocyte differentiation and development of the chloracne-like phenotype by H&E.RESULTS:
In NHEKs, activation of an XRE-luciferase and CYP1A1 protein induction correlated with ligand binding affinity TCDD>ß-NF>ITE. AhR degradation was induced by all ligands. In epidermal equivalents, TCDD induced a chloracne-like phenotype, whereas ß-NF or ITE did not. All three ligands induced involucrin and TGM-1 protein expression in epidermal equivalents whereas FLG protein expression decreased following treatment with TCDD and ß-NF. Inhibition of AhR by α-NF blocked TCDD-induced AhR activation in NHEKs and blocked phenotypic changes in epidermal equivalents; however, AhR knock down did not reproduce the phenotype.CONCLUSION:
Ligand-induced CYP1A1 and AhR degradation did not correlate with their chloracnegenic potential, indicating that neither CYP1A1 nor AhR are suitable biomarkers. Mechanistic studies showed that the TCDD-induced chloracne-like phenotype depends on AhR activation whereas AhR knock down did not appear sufficient to induce the phenotype.Palabras clave
2,3,7,8-tetrachlorodibenzo-p-dioxin; 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester; AHRR; AhR; Aryl hydrocarbon receptor; CYP1A1; Epidermal equivalent; ITE; Keratinocyte; TCDD; TGM-1; XRE; aryl hydrocarbon receptor; aryl hydrocarbon repressor protein; cytochrome P450 1A1; transglutaminase-1; xenobiotic response element; α-NF; α-naphthoflavone; ß-NF; ß-Naphthoflavone; ß-naphthoflavone
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Queratinocitos
/
Receptores de Hidrocarburo de Aril
/
Epidermis
/
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
/
Cloracné
/
Técnicas de Silenciamiento del Gen
/
Dibenzodioxinas Policloradas
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Dermatol Sci
Asunto de la revista:
DERMATOLOGIA
Año:
2014
Tipo del documento:
Article