Comparison of real time polymerase chain reaction with microscopy and antigen detection assay for the diagnosis of malaria.
J Coll Physicians Surg Pak
; 23(10): 787-92, 2013 Nov.
Article
en En
| MEDLINE
| ID: mdl-24169386
ABSTRACT
OBJECTIVE:
To determine the sensitivity of a real time polymerase chain reaction (PCR) for malaria diagnosis and to compare its accuracy with microscopy and an antigen based rapid diagnostic test (OptiMal). STUDYDESIGN:
Cross-sectional analytical study. PLACE AND DURATION OF STUDY Military Hospital, Armed Forces Institute of Transfusion and Armed Forces Institute of Pathology, Rawalpindi, from July to December 2011.METHODOLOGY:
Venous blood samples of 300 clinically suspected patients of malaria were tested for malaria parasite by microscopy and OptiMal; and malaria parasite index was calculated for the positive samples. Plasmodium genus specific real time PCR was performed on all specimens, targeting small subunit rRNA gene. Diagnostic accuracy of three tests was compared and cost analysis was done.RESULTS:
Out of 300 patients, malaria parasite was detected in 110, 106 and 123 patients by microscopy, OptiMAL and PCR respectively. Real time PCR was 100% sensitive while microscopy and OptiMal had sensitivity of 89.4% and 86.2% respectively. All methods were 100% specific. The cost per test was calculated to be 0.2, 2.75 and 3.30 US$ by microscopy, OptiMal and PCR respectively, excluding the once capital cost on PCR equipment.CONCLUSION:
Genus specific real time PCR for the diagnosis of malaria was successfully established as a highly sensitive and affordable technology that should be incorporated in the diagnostic algorithm in this country.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasmodium
/
Pruebas Diagnósticas de Rutina
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Malaria
/
Microscopía
Tipo de estudio:
Diagnostic_studies
/
Health_economic_evaluation
/
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Límite:
Adolescent
/
Female
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Humans
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Male
Idioma:
En
Revista:
J Coll Physicians Surg Pak
Asunto de la revista:
MEDICINA
Año:
2013
Tipo del documento:
Article