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Localization of a highly immunogenic region on the acetylcholine receptor alpha-subunit.
Biochem Biophys Res Commun ; 135(1): 82-9, 1986 Feb 26.
Article en En | MEDLINE | ID: mdl-2420332
Antibodies to synthetic peptides were employed in order to map domains on the alpha-subunit of the acetylcholine receptor to which several monoclonal antibodies are directed. Five peptides corresponding to residues 1-20, 126-143, 169-181, 330-340 and 351-368 of the receptor alpha-subunit were synthesized and antibodies against them were elicited. The anti-peptide antibodies were employed along with the monoclonal antibodies to identify fragments of S. aureus V8 protease digested- alpha-subunit in immunoblotting experiments. Our results demonstrate that a highly immunogenic region of the alpha-subunit is located on a carboxy-terminal 14 kDa portion of the alpha-subunit. This region also seems to undergo antigenic changes during muscle development. A monoclonal antibody directed against the cholinergic binding site of the acetylcholine receptor reacted with an 18 kDa segment of the alpha-subunit which bound alpha-bungarotoxin as well as antibodies directed against peptide 169-181.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1986 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1986 Tipo del documento: Article Pais de publicación: Estados Unidos