Plant regeneration from callus-derived protoplasts of Pelargonium x domesticum.
Plant Cell Rep
; 10(8): 417-20, 1991 Oct.
Article
en En
| MEDLINE
| ID: mdl-24221737
ABSTRACT
A protocol for regenerating plants from callus-derived protoplasts of Pelargonium x domesticum (rega l geranium cv. 'Melissa') has been developed. Protoplasts were isolated from leaf-derived callus tissue on MS medium supplemented with 3.0 mg/l naphthalene acetic acid, 2.0 mg/l 6- benzylaminopurine, and 3.0% sucrose. This callus yielded 2.7×10(5) protoplasts/gram of tissue after a 6 hr incubation in an enzyme solution consisting of 2.0% cellulysin, 0.5% macerase, and 0.5 M sucrose. Protoplasts were plated at 1×10(5) protoplasts/ml in a mixture (1â¶1 v/v) of KMP8/KP liquid medium layered on the same medium solidified with 0.6% agarose. Protoplast division was initiated within 2 days, and colonies of 15 to 50 cells developed 8 wk after plating. P-calli 1-2 mm(3) developed 15 wk after plating, and plants regenerated from the p-calli have been transferred to the greenhouse.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Plant Cell Rep
Asunto de la revista:
BOTANICA
Año:
1991
Tipo del documento:
Article
País de afiliación:
Estados Unidos