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Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
Mariashibu, Thankaraj Salammal; Subramanyam, Kondeti; Arun, Muthukrishnan; Theboral, Jeevaraj; Rajesh, Manoharan; Rengan, Sampath Kasthuri; Chakravarthy, Rajan; Manickavasagam, Markandan; Ganapathi, Andy.
Afiliación
  • Mariashibu TS; Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, India.
Indian J Exp Biol ; 51(10): 849-59, 2013 Oct.
Article en En | MEDLINE | ID: mdl-24266110
Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 microM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 microM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 microM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Técnicas de Embriogénesis Somática de Plantas Idioma: En Revista: Indian J Exp Biol Año: 2013 Tipo del documento: Article País de afiliación: India Pais de publicación: India
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Técnicas de Embriogénesis Somática de Plantas Idioma: En Revista: Indian J Exp Biol Año: 2013 Tipo del documento: Article País de afiliación: India Pais de publicación: India