In vivo fate of avidin-nucleic acid nanoassemblies as multifunctional diagnostic tools.
ACS Nano
; 8(1): 175-87, 2014 Jan 28.
Article
en En
| MEDLINE
| ID: mdl-24328174
This study describes the formulation optimization and body-cell distribution and clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on the novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as a potential advancement of classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely in the bloodstream; it is slowly captured by filter organs; it is efficiently cleared within 24-48 h, and it is poorly immunogenic. The system displays more favorable properties than its parent monomeric avidin and it is a promising tool for diagnostic purposes for future translational aims, for which free circulation in the bloodstream, safety, multifunctionality and high composition definition are all necessary requirements. In addition, the assembly shows a time-dependent cell penetration capability, suggesting it may also function as a NP-dependent drug delivery tool. The ease of preparation together with the possibility to fine-tune the surface composition makes it also an ideal candidate to understand if and how nanoparticle composition affects its localization.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Nucleicos
/
Avidina
/
Nanopartículas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
ACS Nano
Año:
2014
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Estados Unidos