Your browser doesn't support javascript.
loading
Development and validation of an LC-MS/MS method for simultaneous quantitative analysis of free and conjugated bisphenol A in human urine.
Battal, Dilek; Cok, Ismet; Unlusayin, Irfan; Tunctan, Bahar.
Afiliación
  • Battal D; Mersin University, Faculty of Pharmacy, Department of Toxicology, 33169, Mersin, Turkey.
Biomed Chromatogr ; 28(5): 686-93, 2014 May.
Article en En | MEDLINE | ID: mdl-24343900
Bisphenol A (BPA) is an environmental endocrine-disrupting chemicals that is widely used in common consumer products. There is an increasing concern regarding human exposure to BPA owing to the potential adverse effects associated with its estrogenic activity. For assessing environmental exposure to BPA, it is essential to have a sensitive, accurate and selective analytical method, especially one that can detect low BPA levels in complex sample matrices. In this study, we developed and validated an accurate, sensitive, and robust liquid chromatography-tandem mass spectrometry method for simultaneous quantification of free BPA and BPA ß-d-glucuronide (BPA-gluc) concentrations in human urine with only a single injection. Calibration curves were linear over a concentration range of 1-100 ng/mL for BPA and 10-1000 ng/mL for BPA-gluc. The levels of the analytes were determined quantitatively with HPLC/ESI-MS/MS by using negative electrospray ionization in the select ion monitoring mode and a pentaflouraphenyl propyl column. The validated method was applied to the analysis of spot urine specimens collected from randomly selected healthy human subjects.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Compuestos de Bencidrilo / Cromatografía Líquida de Alta Presión / Disruptores Endocrinos / Espectrometría de Masas en Tándem Tipo de estudio: Evaluation_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Biomed Chromatogr Año: 2014 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Compuestos de Bencidrilo / Cromatografía Líquida de Alta Presión / Disruptores Endocrinos / Espectrometría de Masas en Tándem Tipo de estudio: Evaluation_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Biomed Chromatogr Año: 2014 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido