Novel and rapid enumeration method of peripheral blood stem cells using automated hematology analyzer.
Int J Lab Hematol
; 36(5): 521-30, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-24373185
ABSTRACT
INTRODUCTION:
The number of infused CD34(+) cells is crucial to the success of peripheral blood stem cell transplantation (PBSCT). Here, we present, for the first time, a new method of enumerating hematopoietic progenitor cells (HPCs) for PBSCT.METHOD:
This novel method is based on hemolysis and chemical staining, followed by flow cytometry-based optical detection, conducted using an automated hematology analyzer (XN series, Sysmex). CD34(+) cells and HPCs were compared in 76 granulocyte colony-stimulating factor (G-CSF)-mobilized blood or apheresis samples taken from healthy donors (n = 18) or patients undergoing autologous PBSCT (n = 6).RESULTS:
There was a strong correlation between the numbers of HPCs and CD34(+) cells (R(2) = 0.958). The expected total number of HPCs in the final products, which was estimated from HPCs in pre-apheresis PB or mid-apheresis products, also correlated well with the total number of CD34(+) cells in the final products. The change in HPCs in PB closely resembled that of CD34(+) cells during mobilization. Experiments using immunomagnetic beads suggested that the majority of CD34(+) cells existed in HPCs, and vice versa.CONCLUSION:
Hematopoietic progenitor cells may serve as surrogates for CD34(+) cells in PBSCT. However, further investigations are required to verify this.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recuento de Células Sanguíneas
/
Células Sanguíneas
/
Células Madre Hematopoyéticas
/
Neoplasias Hematológicas
/
Trasplante de Células Madre de Sangre Periférica
Tipo de estudio:
Observational_studies
Límite:
Humans
Idioma:
En
Revista:
Int J Lab Hematol
Asunto de la revista:
HEMATOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón