Serological and structural analysis of HLA class I molecules: beta 2-microglobulin interacts with the two external domains of the HLA class I heavy chain.
Ann Inst Pasteur Immunol
; 138(1): 19-35, 1987.
Article
en En
| MEDLINE
| ID: mdl-2437937
ABSTRACT
The serological reactivities of HLA class I molecules were studied in relation to structural modifications of these molecules, including shuffling of external exons and exchange of human beta 2-microglobulin for beta 2-microglobulin from different species. Two major clusters (I and II) of monomorphic and polymorphic antigenic determinants could be delineated. beta 2-Microglobulin participates in the formation of the two clusters, indicating that the light chain interacts tightly with the two external domains of the HLA class I heavy chain. However, external molecules can modify these interactions and alter the antigenic structure of the overall molecule. Thus, fixation on HLA class I molecules of the Fab fragment of a monoclonal antibody directed at antigenic determinants associated with cluster II resulted in enhanced fixation of a monoclonal antibody (B10.6) related to cluster I. The structural and functional implications of these results are discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microglobulina beta-2
/
Antígenos HLA
Límite:
Humans
Idioma:
En
Revista:
Ann Inst Pasteur Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
1987
Tipo del documento:
Article