LIF/STAT3/SOCS3 signaling pathway in murine bone marrow stromal cells suppresses osteoblast differentiation.
J Cell Biochem
; 115(7): 1262-8, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-24464633
ABSTRACT
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin-6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony-forming unit-osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short-hairpin SOCS3-BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Transcripción STAT3
/
Proteínas Supresoras de la Señalización de Citocinas
/
Beta Catenina
/
Factor Inhibidor de Leucemia
/
Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
J Cell Biochem
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón