Influence of probe structure on unique (regiospecific) cleavage of RNA by RNase H.

Metelev, V G; Zayakina, G V; Ryabushenko, I L; Krynetskaya, N F; Romanova, E A; Oretskaya, T S; Shabarova, Z A

Metelev, V G; Zayakina, G V; Ryabushenko, I L; Krynetskaya, N F; Romanova, E A; Oretskaya, T S; Shabarova, Z A.

Afiliación

Metelev VG; Chemistry Department, Moscow State University, USSR.

FEBS Lett ; 226(2): 232-4, 1988 Jan 04.

Article en En | MEDLINE | ID: mdl-2448163

RESUMEN

Chimeric oligo(ribo-deoxyribo)nucleotides with an internucleotide pyrophosphate bond are novel probes for regiospecific hydrolysis of RNA by RNase H. It has been shown that the use of d(TGTGTAT)ppGCCAU leads to unique hydrolysis of the TMV RNA fragment pAAUGGCAUACAC between C10 and A11.

Asunto(s)

Endorribonucleasas/metabolismo; ARN; Secuencia de Bases; Escherichia coli/enzimología; Oligodesoxirribonucleótidos; Oligorribonucleótidos; Ribonucleasa H; Especificidad por Sustrato

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Endorribonucleasas Idioma: En Revista: FEBS Lett Año: 1988 Tipo del documento: Article Pais de publicación: Reino Unido

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