HPLC-fluorescence determination of thiol compounds in the serum of human male and female subjects using HILIC-mode column.
Biomed Chromatogr
; 28(5): 589-93, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24500895
ABSTRACT
High-performance liquid chromatography-fluorescence detection using a hydrophilic interaction chromatography-mode column (ZIC®-HILIC) was used to determine four kinds of thiol compounds in human serum. Sera were obtained from 34 subjects for this study (17 male subjects aged 22-38 years and 17 female subjects aged 18-38 years). Serum cysteine, cysteinylglycine, glutathione, and γ-glutamylcysteine, derivatized with ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate, were separated on the ZIC®-HILIC column and quantified. The serum concentrations of cysteine, cysteinylglycine, glutathione and γ-glutamylcysteine were 226 ± 4.7, 23.4 ± 1.3, 3.7 ± 0.2 and 3.2 ± 0.1 µm, respectively. In addition, the concentrations of serum thiol compounds from male subjects were significantly higher than those of the female subjects (p < 0.05).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cisteína
/
Glutatión
Límite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Biomed Chromatogr
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón