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Measurement of intracellular Ca2+ release in intact cells using 45Ca2+.
Luyten, Tomas; Bultynck, Geert; Parys, Jan B; De Smedt, Humbert; Missiaen, Ludwig.
Afiliación
  • Luyten T; Laboratory of Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine, KU Leuven Campus Gasthuisberg O&N I, 3000 Leuven, Belgium.
Cold Spring Harb Protoc ; 2014(3): 284-8, 2014 Mar 01.
Article en En | MEDLINE | ID: mdl-24591687
This protocol describes a technique using (45)Ca(2+) to measure the release of Ca(2+) from the intracellular stores in monolayers of intact cells cultured in 12-well 4-cm(2) clusters. The (45)Ca(2+)-flux technique described here can only be applied to cell types that adhere to plastic. We describe the loading of the stores with (45)Ca(2+), and the subsequent (45)Ca(2+) efflux.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radioisótopos de Calcio / Calcio Idioma: En Revista: Cold Spring Harb Protoc Año: 2014 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radioisótopos de Calcio / Calcio Idioma: En Revista: Cold Spring Harb Protoc Año: 2014 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos