Measurement of intracellular Ca2+ release in intact cells using 45Ca2+.
Cold Spring Harb Protoc
; 2014(3): 284-8, 2014 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-24591687
This protocol describes a technique using (45)Ca(2+) to measure the release of Ca(2+) from the intracellular stores in monolayers of intact cells cultured in 12-well 4-cm(2) clusters. The (45)Ca(2+)-flux technique described here can only be applied to cell types that adhere to plastic. We describe the loading of the stores with (45)Ca(2+), and the subsequent (45)Ca(2+) efflux.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Radioisótopos de Calcio
/
Calcio
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2014
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
Estados Unidos