NOS1 methylation and carotid artery intima-media thickness in children.
Circ Cardiovasc Genet
; 7(2): 116-22, 2014 Apr.
Article
en En
| MEDLINE
| ID: mdl-24622112
ABSTRACT
BACKGROUND:
Nitric oxide (NO) plays an important role in cardiovascular health by maintaining and regulating vascular tone and blood flow. Epigenetic regulation of NO synthase (NOS), the genes responsible for NO production, may affect cardiovascular disease, including the development of atherosclerosis in children. METHODS ANDRESULTS:
We measured percentage DNA methylation using bisulfite conversion and pyrosequencing assays on DNA from buccal cells provided by 377 participants of the Children's Health Study on whom carotid artery intima-media thickness (CIMT) measurements were also collected. We examined a total of 16 CpG loci located within NOS1, NOS2A, NOS3, ARG1, and ARG2 genes responsible for NO production. CIMT was measured using high-resolution B-mode carotid ultrasound. The association between percentage DNA methylation in ARG and NOS genes with CIMT was evaluated using linear regression adjusted for sex, ethnicity, body mass index, age at CIMT, town of residence, and experimental plate for pyrosequencing reactions. Differences in the association by ethnicity and ancestral group were also evaluated. For a 1% increase in average DNA methylation of NOS1, CIMT increased by 1.2 µm (P=0.02). This association was greater in Hispanic children of Native American descent (ß=2.3; P=0.004) than in non-Hispanic whites (ß=0.3; P=0.71) or Hispanic whites (ß=1.0; P=0.35).CONCLUSIONS:
DNA methylation of NOS1 has a plausible role in atherogenesis through regulation of NO production, although ancestry may alter the magnitude of this association.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arterias Carótidas
/
Aterosclerosis
/
Óxido Nítrico Sintasa de Tipo I
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Circ Cardiovasc Genet
Asunto de la revista:
ANGIOLOGIA
/
CARDIOLOGIA
/
GENETICA MEDICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Canadá